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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
13/04/2011 |
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Data da última atualização: |
13/04/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FERNANDEZ, D.; RAMIRO, D.; MALUF, M. P. |
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Afiliação: |
IRD/CIRAD/INRA; IRD/CIRAD/INRA/IAC; MIRIAN PEREZ MALUF, SAPC. |
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Título: |
Phylogenetic analysis of the WRKY transcription factors gene superfamily in coffee plants. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
WRKY family proteins are transcription factors involved in the regulation of development and plant defense response pathways. The Arabidopsis thaliana WRKY superfamily is made of 75 members. Common to these proteins is a DNA-binding region of approximately 60 amino acids in length which comprises the absolutely conserved sequence motif WRKY adjacent to a novel zinc-finger motif. A comparative phylogenetic analysis of the WRKY gene family in coffee and A. thaliana was conducted to assess the diversity of this family in coffee and to identify homologous coffee genes with putative function in defense responses to pathogens. Bioinformatic analysis of around 200 000 coffee Expressed Sequence Tags (ESTs) identified 313 ESTs with BLAST homologies to WRKY proteins. Almost 30 different putative WRKY genes were obtained, but only 25 unigenes encoding a protein with a WRKY domain were identified. Alignement of the WRKY domain sequences of the 25 coffee unigenes together with those of 72 A. thaliana WRKY genes showed a high conservation of the WRKY motif and the zinc-finger motif in the coffee WRKY domain. The 25 coffee WRKY members were distributed among the 3 main A. thaliana WRKY subgroups, with group I members displaying two WRKY domains, as expected. Conservation of the intron position within the WRKY domain sequence was evidenced when cloning the genomic sequence of one WRKY coffee gene (CaWRKY1). Clustering of the coffee WRKY genes based on the EST distribution in cDNA libraries made from tissues under several physiological conditions allowed to identify genes associated with development or with plant defense responses. To assess the involvement of WRKY genes in the coffee defense response pathways, gene expression patterns are being tested in coffee plants under several defenserelated conditions. MenosWRKY family proteins are transcription factors involved in the regulation of development and plant defense response pathways. The Arabidopsis thaliana WRKY superfamily is made of 75 members. Common to these proteins is a DNA-binding region of approximately 60 amino acids in length which comprises the absolutely conserved sequence motif WRKY adjacent to a novel zinc-finger motif. A comparative phylogenetic analysis of the WRKY gene family in coffee and A. thaliana was conducted to assess the diversity of this family in coffee and to identify homologous coffee genes with putative function in defense responses to pathogens. Bioinformatic analysis of around 200 000 coffee Expressed Sequence Tags (ESTs) identified 313 ESTs with BLAST homologies to WRKY proteins. Almost 30 different putative WRKY genes were obtained, but only 25 unigenes encoding a protein with a WRKY domain were identified. Alignement of the WRKY domain sequences of the 25 coffee unigenes together with those of 72 A. thaliana WRKY genes showed a high conservation of the WRKY motif and the zinc-finger motif in the coffee WRKY domain. The 25 coffee WRKY members were distributed among the 3 main A. thaliana WRKY subgroups, with group I members displaying two WRKY domains, as expected. Conservation of the intron position within the WRKY domain sequence was evidenced when cloning the genomic sequence of one WRKY coffee gene (CaWRKY1). Clustering of the coffee WRKY genes based on the EST distribution in cDNA libraries ... Mostrar Tudo |
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Palavras-Chave: |
Coffee; Protein. |
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Thesagro: |
DNA. |
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Thesaurus Nal: |
Arabidopsis thaliana. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32617/1/Phylogenetic-Analysis.pdf
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Marc: |
LEADER 02492nam a2200181 a 4500
001 1885777
005 2011-04-13
008 2007 bl uuuu u00u1 u #d
100 1 $aFERNANDEZ, D.
245 $aPhylogenetic analysis of the WRKY transcription factors gene superfamily in coffee plants.$h[electronic resource]
260 $aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.$c2007
520 $aWRKY family proteins are transcription factors involved in the regulation of development and plant defense response pathways. The Arabidopsis thaliana WRKY superfamily is made of 75 members. Common to these proteins is a DNA-binding region of approximately 60 amino acids in length which comprises the absolutely conserved sequence motif WRKY adjacent to a novel zinc-finger motif. A comparative phylogenetic analysis of the WRKY gene family in coffee and A. thaliana was conducted to assess the diversity of this family in coffee and to identify homologous coffee genes with putative function in defense responses to pathogens. Bioinformatic analysis of around 200 000 coffee Expressed Sequence Tags (ESTs) identified 313 ESTs with BLAST homologies to WRKY proteins. Almost 30 different putative WRKY genes were obtained, but only 25 unigenes encoding a protein with a WRKY domain were identified. Alignement of the WRKY domain sequences of the 25 coffee unigenes together with those of 72 A. thaliana WRKY genes showed a high conservation of the WRKY motif and the zinc-finger motif in the coffee WRKY domain. The 25 coffee WRKY members were distributed among the 3 main A. thaliana WRKY subgroups, with group I members displaying two WRKY domains, as expected. Conservation of the intron position within the WRKY domain sequence was evidenced when cloning the genomic sequence of one WRKY coffee gene (CaWRKY1). Clustering of the coffee WRKY genes based on the EST distribution in cDNA libraries made from tissues under several physiological conditions allowed to identify genes associated with development or with plant defense responses. To assess the involvement of WRKY genes in the coffee defense response pathways, gene expression patterns are being tested in coffee plants under several defenserelated conditions.
650 $aArabidopsis thaliana
650 $aDNA
653 $aCoffee
653 $aProtein
700 1 $aRAMIRO, D.
700 1 $aMALUF, M. P.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
13/08/2001 |
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Data da última atualização: |
27/09/2004 |
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Autoria: |
FERREIRA, A. de M.; TORRES, C. A. A. |
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Título: |
Perda de Peso Corporal e Cessacao da Atividade Ovariana Luteinica Ciclica em Vacas Mesticas Leiteiras. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.28, n.3, p.411-418,mar.1993 |
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Idioma: |
Português |
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Conteúdo: |
Estudou-se o efeito da alimentacao na atividade ovariana luteInica ciclica (AOLC) de 30 vacas mestiCas HolandEs x Zebu, nao-lactantes, multiparas, com peso medio de 535,5 ± 48,4 kg, e boa ou muito boa condicao corporal (escore 4 ou 5, na escala de 1 a 5). Esses animais foram sorteados em dois grupos: I) dez vacas alimentadas para manter o peso inicial, e II) vinte vacas submetidas a subnutricao ate cessacao da AOLC. Semanalmente, foram efetuadas: pesagem dos animais, avaliacao do escore, colheita de sangue (para dosagem de progesterona-RIA) e avaliacao da AOLC (niveis de progesterona, exame ginecologico e observacao visual do estro). No grupo II (vacas subnutridas), o anestro ocorreu em 183,5 ± 30,3 dias, apos os animais perderem, em media, 35,7 ± 3,8% do peso inicial e pesarem 344,5 ± 25,3 kg de peso vivo e escore medio de 2,2 ± 0,3. No grupo I, todas as vacas continuaram exibindo ciclos estrais regulares durante o experimento. Conclui-se que vacas de escore 4 ou 5 necessitam perder muito do peso corporal para que a AOLC seja afetada. |
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Thesagro: |
Bovino; Condição Corporal; Corpo Lúteo; Nutrição. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/20502/1/pab93_17_mar.pdf
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Marc: |
LEADER 01608naa a2200181 a 4500
001 1105405
005 2004-09-27
008 1993 bl uuuu u00u1 u #d
100 1 $aFERREIRA, A. de M.
245 $aPerda de Peso Corporal e Cessacao da Atividade Ovariana Luteinica Ciclica em Vacas Mesticas Leiteiras.
260 $c1993
520 $aEstudou-se o efeito da alimentacao na atividade ovariana luteInica ciclica (AOLC) de 30 vacas mestiCas HolandEs x Zebu, nao-lactantes, multiparas, com peso medio de 535,5 ± 48,4 kg, e boa ou muito boa condicao corporal (escore 4 ou 5, na escala de 1 a 5). Esses animais foram sorteados em dois grupos: I) dez vacas alimentadas para manter o peso inicial, e II) vinte vacas submetidas a subnutricao ate cessacao da AOLC. Semanalmente, foram efetuadas: pesagem dos animais, avaliacao do escore, colheita de sangue (para dosagem de progesterona-RIA) e avaliacao da AOLC (niveis de progesterona, exame ginecologico e observacao visual do estro). No grupo II (vacas subnutridas), o anestro ocorreu em 183,5 ± 30,3 dias, apos os animais perderem, em media, 35,7 ± 3,8% do peso inicial e pesarem 344,5 ± 25,3 kg de peso vivo e escore medio de 2,2 ± 0,3. No grupo I, todas as vacas continuaram exibindo ciclos estrais regulares durante o experimento. Conclui-se que vacas de escore 4 ou 5 necessitam perder muito do peso corporal para que a AOLC seja afetada.
650 $aBovino
650 $aCondição Corporal
650 $aCorpo Lúteo
650 $aNutrição
700 1 $aTORRES, C. A. A.
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.28, n.3, p.411-418,mar.1993
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Embrapa Unidades Centrais (AI-SEDE) |
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